Moorella thermoacetica temperature

(A) shows the schematic representation of the constructed plasmids and the homologous recombination events in the pyrF or pduL2 region. Nov 9, 2022 · A hallmark of the genus Moorella is acetogenic growth with H 2 /CO 2 and/or CO/CO 2 via the Wood–Ljungdahl pathway. Moorella thermoacetica and Moorella thermoautotrophica (formerly Clostridium thermoaceticum and C. 0 NaHCO 3, 10 ml/L trace elements, 10 ml/L Wolfe's vitamin Dec 1, 2000 · Moorella thermoacetica is a significant problem in the canned food industry because its spores are the most Temperature and ethanol are known to perturb the degree of order within membranes Moorella thermoacetica is one of the model acetogenic bacteria for the resolution of the Wood-Ljungdahl (acetyl-CoA) pathway in which CO2 is autotrophically assimilated yielding acetyl-CoA as central intermediate. Moorella thermoacetica, previously known as Clostridium thermoaceticum, is an acetogenic, thermophilic, strictly anaerobic, endospore-forming, bacterium belonging to the phylum Bacillota. Dec 1, 2004 · The ability of M. thermoacetica grows at a temperature higher than the boiling point of acetone (58 °C); therefore, thermophilic processes of gas fermentation producing volatile chemicals could be built and evaluated. 2012) Ralstonia eutropha Jan 4, 2022 · growth temperature (55 C) or suboptimal temperature (45 C), with four batches of each. The thermophilic characteristic of M. On the contrary, strain DSM 21394, currently still named M. Strain SLA38 T is capable of acetogenic growth on CO with and without an electron acceptor. (B) shows the agarose gel electrophoresis following PCR amplification of the pyrF or pduL2 Abstract. For ET-5b, the Sep 10, 2008 · Of these, 1834 genes (70. [1] Researchers at the Lawrence Berkeley National Laboratory , including Peidong Yang , were able to induce M. For the assays, a temperature of 45°C was routinely chosen because the handling of the anoxic cuvettes at 45°C was easier than at 55°C, the growth temperature optimum of M. The 16S rRNA gene sequence of ET-5a was 99. thermoacetica genome can be grouped in orthologue clusters. Jan 4, 2022 · The bacterium Moorella thermoacetica produces the most heat-resistant spores of any spoilage-causing microorganism known in the food industry. thermoacetica can also use dimethyl sulfoxide as terminal electron acceptor. The possible electron transport chain in Moorella is summarized in Fig. For M. Front Microbiol 2020; 10 :3070. Sep 1, 2019 · 150 rpm and a temperature of 55 Moorella thermoacetica , a model acetogenic bacterium, has attracted significant attention due to its ability to utilize carbon dioxide (CO 2 ) and carbon Jun 1, 2016 · Moorella thermoacetica, a gram-positive, thermophilic, and anaerobic bacterium (previously known as Clostridium thermoacetica), is a homoacetogen in which acetate is the major product of its metabolic pathway . As a thermophilic 45‒65 bacterium, M. thermoacetica, obviously represents a new Moorella species. Moorella thermoacetica (a gram-positive thermophilic, anaer- 150 rpm and a temperature of 55 C. Download scientific diagram | Ultrastructure of spores of Moorella thermoacetica ATCC 39073 produced at 55 • C (A) or 45 • C (B) on agar plates, observed by transmission electron microscopy. thermoacetica to use nitrate as an elec- tron acceptor and to consume and tolerant small amounts of O 2 indicate that this bacterium can accommodate a wide May 15, 2024 · DNA construction for introducing bsALDC into the chromosome of Moorella thermoacetica Y72 by homologous recombination. Gas fermentation is one of the promising bioprocesses to convert CO 2 or syngas to important Lower Temperature. This bacterium has a Acetyl-CoA pathway and methanogenesis. coli by inducing the expressions of Moth_1671 and 1672, putative R and M subunits of a Type I R–M system, respectively, was increased to (8. In this work, the effects of sparged CO2 as well as sparged and non-sparged N2 on these microorganisms were studied using glucose and cellobiose as substrates. Feb 10, 2014 · We determined a draft genome sequence for Moorella thermoacetica strain Y72, a syngas-assimilating bacterium with high transformation efficiency. thermoacetica reduces the risk of bacterial contamination during its fermentation (Kato et al. thermoacetica. The G+C content of the DNA was 57. A thermophilic, fermentative microaerophile (ET-5b) and a thermophilic acetogen (ET-5a) were coisolated from oxic soil obtained from Egypt. 8 . 2021). , 2017 ; Rahayu et al Jun 1, 2021 · This work indicated that copper nanoparticles (Cu NPs) were able to cause cell membrane oxidative damage and inhibit the growth and metabolism of Moorella thermoacetica (a model acetogen). 8, but a pH range of 5. Since M. 1992). Innate properties of en-dospores, such as moisture content, dipicolinic acid con- The bacterium Moorella thermoacetica produces the most heat-resistant spores of any spoilage-causing microorganism known in the food industry. Kinetic analysis indicated the presence Moorella thermoacetica is one of the well-studied thermophilic acetogenic bacteria. Here, we used electron microcopy to characterize the ultrastructure Jan 17, 2020 · Despite genetic similarities, differences in genomic features were observed between the strains. A metabolic pathway related to acetone reduction was selected, and acetone conversion to IPA was achieved via the heterologous expression of secondary alcohol dehydrogenase ( sadh ) in the thermophilic bacterium. RAGSDALE* Department of Biochemistry, Beadle Center, University of Nebraska—Lincoln, Lincoln, Nebraska 68588-0664 The development of the engineered thermophilic acetogen M. 3 to 0. 1 KCl, 0. Figure 1. thermoacetica on glucose or H 2 + CO growth temperature ofBacillus strains and the heat resis-tance of their spores (Warth 1978). 1% (wt/vol) yeast extract, formed unusually heat-resistant spores. 1954; Condon et al. G. 5°C. 2018 ). thermoacetica (at 55°C, you burn your fingers). Its metabolic diversity and the ability to efficiently convert a wide range of compounds, including syngas Feb 1, 2020 · Moorella thermoacetica (a gram-positive thermophilic, anaerobic bacterium, previously known as Clostridium thermoaceticum) is a homoacetogen in which acetate is the major product of its metabolic pathway (8). 02 CaCl 2 ·2H 2 O, 2. Jul 1, 2001 · O-Demethylase from Moorella thermoacetica DEVENDRA NAIDU AND STEPHEN W. homoacetogen M. 002 Corpus ID: 259967393; Moorella thermoacetica: A Promising Cytochrome- and Quinone-Containing Acetogenic Bacterium as Platform For A CO2-based Bioeconomy Mar 12, 2012 · M. , 1942) for elucidating the WoodLjungdahl pathway. This acetogen is robust, with a wide range of growth temperatures (45–65°C). Nov 10, 2014 · M. MtvB catalyzes methyl transfer from a phenylmethylether to the cobalt center of Sep 17, 2004 · The gram-positive, thermophilic, acetogenic bacterium Moorella thermoacetica can reduce CO 2 to acetate via the Wood-Ljungdahl (acetyl coenzyme A synthesis) pathway. 1 g of KH 2 PO 4, 0. 2 g of MgSO 4 ·7H 2 O, 0. 7% of the control, respectively series of M. Two strains of Moorella thermoacetica, JW/DB-2 and JW/DB-4, isolated as contaminants from autoclaved media for chemolithoautotrophic growth containing 0. Moorella thermoacetica 24-1 (our collection) + + + + + + was generated after the last amplification cycle using a temperature range of 64 to 95°C and a temperature transition rate of 0. 0 ± 1. thermoacetica utilizes thiosulfate, and the molecular details would be as follows. Genome-Based comparison of all species of the genus Moorella, and status of the species Moorella thermoacetica and Moorella thermoautotrophica. When grown on glucose, these organisms produce three molecules of acetate per glucose and little CO 2. , 2017). 7 is tolerated. ther-moacetica reduces the risk of bacterial contamination. The Moorella thermoacetica est une bactérie d’altération anaérobie thermophile sporulée produisant les spores les plus thermorésistantes isolées à ce jour en industrie agroalimentaire. 1) × 10 1 Here the effect of temperature on MES by the acetogenic thermophiles Moorella thermoacetica and Moorella thermoautotrophica is investigated. To date, very limited genetic manipulation has been achieved in the thermophilic acetogen, Moorella thermoacetica, partly due to the lack of suitable shuttle vectors Jun 19, 2017 · Fermentation with acetogens can be affected by cultivation gas phase, but to date, there is not enough evidence on that matter for Clostridium thermocellum and Moorella thermoacetica. Experiments were performed at operating temperatures ranging from 25 °C to 70 °C to determine the optimum operating temperature for MES. RESULTS. t, ATCC39073) was obtained from the Global Bioresource Center ATCC, and inoculated into 50 mL of heterotrophic medium at 52 °C with a ratio of 1:5 (Table S9). The pH optimum is 6. Also, a correlation be-tween temperature of sporulation and heat resistance of spores has been observed (Khoury et al. Feb 22, 2013 · Moorella thermoacetica was long the only model organism used to study the biochemistry of acetogenesis from CO2. Further analyses confirmed that ET-5a was a new strain of M. A total of 2384 (91. HUC-221, a relative of M. 22, which was modified from [322]. May 12, 2022 · Bacterial Strains and Growth Conditions. thermoacetica, three genes, namely Moth_0450, Moth_0451, and Moth_0452, are homologous to the thiosulfate reductase complex PhsABC from Salmonella enterica (52). thermoacetica is a robust and versatile heterotroph with optimum growth conditions of 55–60 °C and pH 6. thermoacetica microbial cell suspension (OD 600 of 0. Jan 28, 2024 · Moorella thermoacetica, a thermophilic acetogenic microorganism, was genetically engineered to produce IPA. Acetogens: biochemistry, bioenergetics, genetics, and biotechnological potential. The ability of M. Strain Y72 is a strain related to the type strain ATCC 39073 and has been reported to have a less stringent restriction-modification system, which could alleviate the cumbersome transformation process. Temperatures encountered in cannery allow growth of thermophilic spore-forming bacteria, including the strictly anaerobe Moorella thermoacetica, which grows optimally from 55 °C to 65 °C and is the main cause of spoilage of low-acid canned foods (LACFs) at high temperature. 92%) had no database match. 2021 ). Jun 12, 2017 · An interesting heterologous product candidate is for example acetone. Moorella thermoacetica Stephanie Redl 1*, Sumesh Sukumara 1, Tom Ploeger 2, Liang Wu 2, Torbjørn Ølshøj Jensen 1, at an elevated temperature (optimum 55 60 °C []), 7 Moorella thermoacetica is one of the well-studied ther-mophilic acetogenic bacteria. Although further study would be needed to improve the productivity for realization of the industrial applications, the gas fermentation Sep 15, 2023 · Moorella thermoacetica, a model acetogenic bacterium, has attracted signi … In the context of the rapid development of low-carbon economy, there has been increasing interest in utilizing naturally abundant and cost-effective one-carbon (C1) substrates for sustainable production of chemicals and fuels. Jul 1, 2023 · DOI: 10. 17%) of the ORFs in the M. 2016) Ralstonia eutropha H16: Expresses electricity-driven bioconversion of CO 2 to isobutanol and 3-methyl-1-butanol (Li et al. greenca. Nov 1, 1999 · A thermophilic, fermentative microaerophile (ET-5b) and a thermophilic acetogen (ET-5a) were coisolated from oxic soil obtained from Egypt. Redl S, Poehlein A Apr 1, 2013 · However, the genome of Moorella thermoacetica has already been sequenced (11), the physiology has been well characterized (12), and we recently reported that Moorella sp. Sep 1, 2023 · This thermophilic acetogen grows at temperatures of 45–65 °C, but has its optimal growth at a temperature of 55–65 °C. May 1, 2017 · A transformant of Moorella thermoacetica was constructed for thermophilic ethanol production from lignocellulosic biomass by deleting two phosphotransacetylase genes, pdul1 and pdul2, and (DaPFOR) (15–17), the OOR from Moorella thermoacetica (MtOOR) (12, 13), and two OFORs from Sulfolobus tokodaii (StOFOR), the physiological substrate of which has not been established (18). 8 NaCl, 0. Dec 7, 2017 · A multiplex PCR assay was developed to simultaneously detect the thermophilic spore-forming bacteria Geobacillus stearothermophilus and Moorella thermoacetica, which are major sources of spoilage in the canned food and beverage industries because of their thermophilic persistence. Extremophiles 20, 653–661. thermoacetica ATCC39073 using an E. M. By adding H2 to Formyltetrahydrofolate synthetase (FTHFS) from the thermophilic homoacetogen, Moorella thermoacetica, has an optimum temperature for activity of 55-60 degrees C and requires monovalent cations for both optimal activity and stabilization of tetrameric structure at higher temperatures. . Oct 28, 2022 · Replicating plasmid shuttle vectors are key tools for efficient genetic and metabolic engineering applications, allowing the development of sustainable bioprocesses using non-model organisms with unique metabolic capabilities. 2 MgSO 4 ·7H 2 O, 0. thermoacetica in the presence of 1 mg/L of Cu NPs were decreased to 29. Feb 10, 2014 · In our study, compared with using a non-methylated shuttle vector, the transformation efficiency of M. thermoacetica normally grows within 45‒65 °C, with an optimum temperature of 55–60 °C. Dec 12, 2007 · To elucidate the role of CBS domains in family II PPases, we have cloned, expressed and purified a CBS-PPase from Moorella thermoacetica (formerly known as Clostridium thermaceticum) (mtCBS-PPase), a low-G+C Gram-positive thermophilic acetogen with an optimum growth temperature of 55–60 °C . 2020; Sakai et al. thermoautotrophica, methylenetetrahydrofolate reductase and CO dehydrogenase could be linked to energy-conserving steps in the Wood–Ljungdahl pathway. Its further conversion into acetate relies on subsequent phosphotransacetylase (PTA) and acetate kinase reactions. Here, we describe that M. Apr 23, 2021 · Our development of the engineered thermophilic acetogen M. Growth of M. A transformant of Moorella thermoacetica was constructed for thermophilic ethanol production from lignocellulosic biomass by deleting two phosphotransacetylase genes, pdul1 and pdul2, and introducing the native aldehyde dehydrogenase gene (aldh) controlled by the promoter from glyceraldehyde-3-phosphate dehydrogenase. 2005; Wang and Wang 1984). , 2009; Abdel-Banat et al. 2023. In the genome of M. 43%) matched genes of unknown function, and the remaining 24 (0. Growth with Dec 22, 2005 · A thermophilic, fermentative microaerophile (ET-5b) and a thermophilic acetogen (ET-5a) were coisolated from oxic soil obtained from Egypt. 0 g of NH 4 Cl, 0. thermoacetica AMP and M. A total of 294 samples were examined, and none of the samples Temperatures Kind of temperature Temperature [Ref. Moorella thermoacetica grows at 45 °C–65 °C. Spores of the two strains required heat activation at 100 °C of more than 2 min and up to 90 min for maximal percentage of germination. We also developed a strategy to increase the carbon ux to ace-tone by genetic engineering and evaluated the productiv-ity from CO 2 –H 2, CO and CO–H 2 as a model syngas. thermoacetica uses a wide variety of electron donors and acceptors Oct 27, 2023 · Moorella thermoacetica (formerly Clostridium thermoaceticum) has been the model bacterium to unravel the WLP and the enzymes involved [[1, 2, 16, 17]]. 1 KH 2 PO 4, 0. We determined the genome sequence of Moorella thermoacetica, originally named Clostridium thermoaceticum, which has been the model acetogen since its isolation in 1942 (Fontaine et al. 2% and 40. thermoacetica strain ATCC39073. The bacterium reduced perchlorate A total of 2384 (91. thermoacetica grows at an elevated temperature (optimum 55–60 °C ), products with low boiling point such as acetone (boiling point 56 °C ) would allow for easy, inexpensive product May 21, 2015 · Moorella thermoacetica (formerly Clostridium thermoaceticum 8) is a strictly anaerobic, spore-forming, thermophilic, Gram-positive acetogen that has been extensively studied as a model organism for over 50 years to identify and characterize the enzymes of WLP. stamsii hydrogenogenic growth on CO/CO 2 was shown (Jiang et al. coli–Moorella shuttle vector methylated in E. 8 g of NaCl, 0. 06. The bacterium Moorella thermoacetica produces the most heat-resistant spores of any spoilage-causing microorganism known in the food industry. Jun 1, 2001 · The Moorella thermoacetica aromaticO-demethylase was characterized as an inducible three-component system with similarity to the methanogenic methanol, methylamine, and methanethiol methyltransferases and to theO-demethylase system from Acetobacterium dehalogenans. 0 g of NaHCO 3, 10 ml of trace elements, 10 ml of Wolfe’s vitamin solution (Tanner, 1989), and 1. 0 mg Jan 28, 2024 · Moorella thermoacetica, a thermophilic acetogenic microorganism, was genetically engineered to produce IPA. 8% similar to that of the classic acetogen Moorella thermoacetica. This first genome sequence of an acetogenic bacterium provides Jul 1, 2023 · M. thermoacetica can use a variety of carbon sources such as CO 2, CO, monosaccharides, organic acids, alcohols or methoxylated aromatic compounds [83]. Spores of Moorella thermoacetica strain ATCC 39073, produced on agar plates at Dec 1, 2017 · Moorella thermoacetica ATCC 39073 (American Type Culture Collection, Rockville, MD) and its derivatives (Table 1) were grown in basal medium modified from ATCC 1754 PETC medium containing the following compounds in deionized water (as g/L): 1. The crystal structures of complexes of FTHFS with cesium and potassium ions were examined and monovalent cation Jan 28, 2024 · Results Moorella thermoacetica , a thermophilic acetogenic microorganism, was genetically engineered to produce IPA. On the basis of 16S rRNA analysis, strain An10 was most closely related to Moorella thermoacetica and Moorella thermoautotrophica. thermoacetica on glucose or H 2 + CO 2 was stimulated Sep 15, 2023 · The development of the engineered thermophilic acetogen M. , 2008). Apr 1, 2023 · The development of the engineered thermophilic acetogen M. thermoacetica strains for thermophilic acetone production. thermoacetica is a Gram-positive, endospore-forming, and cytochrome-containing anaerobic bacterium that can grow on glucose, forming three acetic acids (reactions 5 to 7) , or on H 2 and CO 2 (reactions 8 and 9) (12, 34) with a growth temperature optimum near 55°C : May 15, 2024 · Two propanediol utilization-like proteins of Moorella thermoacetica with phosphotransacetylase activity. 4) was serially diluted (10 8 to 10 1 CFU/ml) and then spiked into Moorella-free microbial pellets derived from the canned coffee beverages. Depending on the growth substrate, this Gram-positive bacterium can either form H2 Apr 21, 2021 · The development of the engineered thermophilic acetogen M. high temperatures become superior to the traditional process using Publication: Redl S, Poehlein A, Esser C, Bengelsdorf FR, et al. Abstract: The bacterium Moorella thermoacetica produces the most heat-resistant spores of any spoilage-causing microorganism known in the food industry. Differences in compounds that can serve as carbon and energy sources for selected strains were also identified. , 1942). 10. 1987; Williams et al. 0 NH 4 Cl, 0. thermoacetica is a Gram-positive, motile, endospore-forming thermophile with an optimal growth temperature of 55–60 °C and is a common inhabitant of soils 2. This first genome sequence of an acetogenic bacterium provides important information related to how acetogens engage their extreme metabolic diversity by switching among different carbon substrates and electron donors/acceptors and how Pierce et al. thermoacetica normally grows within °C, with an optimum temperature of 55–60 °C. 2,9–14 M. Aug 1, 2018 · Temperatures encountered in cannery allow growth of thermophilic spore-forming bacteria, including the strictly anaerobe Moorella thermoacetica, which grows optimally from 55 °C to 65 °C and is the main cause of spoilage of low-acid canned foods (LACFs) at high temperature. , 2005a) by the batch method in melting point capillaries (at room temperature) by mixing 10 μ l of ≈1 m M (≈42 mg/ml) oxidized (as‐isolated) (Mt_FDP_ox) solution to an equal volume of precipitant solution. We found that, unlike all other known PPases, this The bacterium Moorella thermoacetica produces the most heat-resistant spores of any spoilage-causing microorganism known in the food industry. thermoacetica, produced ethanol besides acetate from H 2 plus CO 2 13, 14. thermoacetica strains have been identied (Table 1), in which some have received extensive concern (Redl et al. 7–7. Jan 4, 2022 · Insights into the Structure and Protein Composition of Moorella thermoacetica Spores Formed at Different Temperatures. To our knowledge, this is the rst study to provide strains for Nov 3, 2022 · In addition, Moorella thermoacetica (M. thermoacetica to produce acetone from gaseous substrates at high temperature. Moorella thermoacetica ferments glucose to three acetic acids. It grows by oxidation of organic substrates, CO or H 2 coupled to CO 2 reduction to acetate. The most well-studied OFOR is the one described here, the PFOR from the model acetogen M In M. The OD 600 and acetic acid production of M. ther- The flavodiiron protein from Moorella thermoacetica (Mt_FDP) was crystallized ( Silaghi‐Dumitrescu et al. thermoacetica contains a membrane-bound cytochrome bd oxidase that can catalyze reduction of low levels of dioxygen. In the oxidative part of the fermentation, the hexose is converted to 2 acetic acids and 2 CO2 molecules with the formation of 2 NADH Nov 20, 2012 · a boiling temperature lower than culture broth, thereby enabling. (2020), this species is an earlier heterotypic synonym of Moorella Nov 2, 2007 · The isolate was able to respire with (per)chlorate, nitrate, thiosulfate, neutralized Fe(III) complexes, and anthraquinone-2,6-disulfonate. Despite show-casing the potential in the utilization of C1 gases, natural. ⏰ According to Redl et al. Previous work by our group revealed that the resistance of these spores to wet heat and biocides was lower when spores were produced at a lower temperature than the optimal temperature. 1007/s00792-016-0854-6 [Google Scholar] Debabov V. A pathway for thermophilic acetone production, which functions up to 70 °C, has been proposed with enzyme candidates (Zeldes et al. These cases are limited to the production of C2 and C3 compounds, such as ethanol (C2), lactate, acetone, and isopropanol (C3) ( Iwasaki et al. Apr 29, 2024 · This brief report attempts to expand the product spectrum to include C4 compounds by using strain Y72 of Moorella thermoacetica. thermoacetica can be used to establish an advantageous bioprocess for the recovery of products, especially volatile chemicals (Taylor et al. The taxonomy of the genus Clostridium was restructured in 1994 and C. thermoacetica and M. thermoaceticum was transferred to a new genus Moorella as M. This report demonstrates that, despite its classification as a strict anaerobe, M. thermoacetica spores to survive high temperatures demonstrates that this anaero- bic bacterium can also survive standard commercial canning procedures. 2013). e thermophilic characteristic of M. 13%) have been assigned tentative functions, 665 (25. Apr 23, 2021 · M. 1 g of KCl, 0. The recombinant enzyme was found to have a broader temperature range and greater stability, at both elevated and low temperatures, than previously described cyanuric acid hydrolases. 2009; Alves et al. thermoacetica (Collins et al May 14, 2024 · To date, the most successful cases of metabolic engineering to produce other chemicals have been achieved using the model thermophilic acetogen Moorella thermoacetica ATCC 39073. thermoacetica because its overall genome sequence characteristics were similar to those of M. Moorella thermoacetica ATCC 39073 and its derivatives were used in this study (Table 1). Modified ATCC1754 PETC medium comprising 1. These pellets were prepared prior to confirmation of a negative result in the real-time PCR assay with a 40-cycle amplification. Slurry samples of each lignocellulose biomass Jun 20, 2022 · Moorella thermoacetica: Demonstrates a self-replicating pathway toward light-to-chemical CO 2 reduction by selectively producing acetic acid from CO 2 (Sakimoto et al. Resistance to wet-heat, biocides and UV-C of spores formed May 15, 2024 · This brief report attempts to expand the product spectrum to include C4 compounds by using strain Y72 of Moorella thermoacetica. Previous work by our group revealed that the resistance of these spores to wet heat and biocides was lower when spores were produced at a lower temper … Apr 23, 2021 · Design and construction of genetically engineered M. Sep 15, 2023 · As a thermophilic bacterium, M. Page 2 NIH-PA Author Manuscript “homoacetogen” and “CO2-reducing acetogen”. thermoacetica to photosynthesize, despite its Jul 1, 2015 · Next, 1 ml of M. during its fermentation (Kato et al. Thiosulfate reductase produces hydrogen sulfide (H 2 S) and sulfite. It was revealed that sparged CO2 and non-sparged N2 Moorella thermoacetica is a strictly anaerobic, endospore-forming, and metabolically versatile acetogenic bacterium capable of conserving energy by both autotrophic (acetogenesis) and heterotrophic (homoacetogenesis) modes of metabolism. Spores produced at 55 C appeared spherical and highly heterogeneous in terms of size (Figure1A). 02 g of CaCl 2 ·2H 2 O, 2. 6 mol%. (2021). As a thermophilic bacterium, M. In a few cases, however, the measurements were done at 55°C, and at this temperature, the activity was found to be twice Formyltetrahydrofolate synthetase (FTHFS) from the thermophilic homoacetogen, Moorella thermoacetica, has an optimum temperature for activity of 55−60 °C and requires monovalent cations for both optimal activity and stabilization of tetrameric structure at higher temperatures. May 11, 2022 · Moorella thermoacetica is a thermophilic acetogen (Drake and Daniel, 2004; Pierce et al. Very few family members have been extensively characterized. The species was isolated from horse feces in 1942 and named Clostridium thermoaceticum (Fontaine et al. thermoacetica, which grows at a temperature higher than the boiling point of acetone (58 °C), would pave the way for developing a consolidated process with simplified and cost-effective recovery via condensation following gas fermentation. Due to its thermophilic nature, M. , 2010; Basen and Müller, 2017; Redl et al. They were then observed by SEM (n = 78 and n = 73 spores, respectively). : #36735] and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica. 8% identical to that of the classic acetogen Moorella thermoacetica. thermoautotrophicum) are facultative autotrophs and obligately anaerobic spore formers. This strain was confirmed to be M. 1016/j. Dec 1, 2017 · Temperatures encountered in cannery allow growth of thermophilic spore-forming bacteria, including the strictly anaerobe Moorella thermoacetica, which grows optimally from 55 °C to 65 °C and is Jan 16, 2020 · A well-studied syngas-fermenting thermophile is Moorella thermoacetica. The potential advantage was tested using engineered strains of Moorella thermoacetica that produce ethanol. The formate dehydrogenase and methylene-THF dehydrogenase use NADPH as reductant, whereas the putative electron-bifurcating methylene-THF reductase uses NADH as electron donor and an unknown Sep 18, 2009 · In this study, cyanuric acid hydrolase from the thermophile Moorella thermoacetica ATCC 39073 was cloned, expressed in Escherichia coli, and purified to homogeneity. kp wj ui xu zu no gc ls km ow